The RealBest diagnostic kits are intended for the detection of infectious agents and determination of genetic polymorphisms by real-time PCR. In-house developed technologies turn the procedure of real-time PCR analysis from a complicated process requiring extensive specialized knowledge and practical experience into a standardized, simple, and readily reproducible procedure. AO Vector-Best offers user-friendly solutions for diagnostics such as lyophilized ready-to-use mixtures with a long shelf-life and transportation temperature of up to 26 °С.
Str-format: Ready Master Mix is lyophilized in 0.2 ml white tubes of the 96-tube plate. The only step required for the reaction procedure is the addition of extracted DNA/RNA samples
Uni-format:Ready Master Mix is lyophilized in clear 0.2 ml tubes. Add the extracted DNA/RNA into the tubes and run the test. One tube – one reaction
Str and Uni formats can be used with: CFX96™ (Bio-Rad, USA), DTprime, DT-96 (DNA-Technology, Russia), Rotor-Gene® Q (Qiagen, Germany), Rotor-Gene® 6000/3000 (Corbett Research, Australia)
|Number of simultaneously detected pathogens||Detection channels|
|2||FAM, ROX, HEX (JOE)|
|3 and more||FAM, ROX, HEX (JOE), Cy5|
Other real-time PCR cyclers with similar technical characteristics can be used after validation